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To investigate whether ROS was host or pathogen generated, a separate experiment was conducted in which cells were infected with 1 MOI zoospores (T#5), and either: 1) supplemented with 2.5 mM cysteine on day 3 for 8 hr, 2) incubated at 27°C for 8 hr, 3) supplemented with 1.5 mM cysteine and incubated at 30°C for 6 hr
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Cleaved Caspase 3 (CC3) is a biomarker which signifies cell death by apoptosis, and it is an indicator of the loss of cardiomyocytes that cause damage to the myocardium
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