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Cells in the control and experimental groups were clustered into 25 different clusters by the UMAP algorithm, and the 25 clusters were categorized into seven different cell types in prostate cancer cells based on the gene expression of different clusters (Figure 8A and Tables S13–S15) We further analyzed the cellular expression characteristics of the pathway genes and found that the pathway genes were significantly differentially expressed between the tumor and normal groups (Figure 8B)

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