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The COS7 cellular background was used owing to the absence or low expression of endogenous RAMPs, , and, as such, it provided the best available system for testing the human CTR (hCTR) and different heterodimeric amylin receptor subtypes functionally generated by the introduction of the individual RAMPs

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This gender disparity may relate to multiple factors including sex hormone levels, differences in gut motility, psychosocial factors, and healthcare-seeking behavior