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After excluding lineage-positive cells using BioMag-streptavidin (BD Biosciences) and biotin-conjugated anti-CD4, -CD8, -B220, -Mac1, -Gr1, and -Ter119 antibodies (Pharmingen), cells from E13.5 fetal livers of Gata1 ΔNeoΔHS/Y embryos were expanded with a combination of thrombopoietin (10 ng/ml), stem cell factor (50 ng/ml), and interleukin-3 (IL-3
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