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Cells were lysed in a buffer containing 6 M urea, 2 M thiourea, 50 mM ammonium bicarbonate, 1 mM DTT, Complete Protease Inhibitor (Roche), and 2 mM PMSF
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The importance of S-glutathionylation as a post-translational modification in modulating cellular processes is underscored by the ubiquity of these reactions in mammalian cells
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