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120 In myeloid cell-specific SIRT1 knockout mice, lipid toxicity or LPS stimulation induced more severe hepatic inflammation, elevated pro-inflammatory cytokine levels, and enhanced M1 macrophage polarization, highlighting the critical role of SIRT1 in preserving immune balance
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For RNAi experiments, eggs were isolated by treating adult hermaphrodites with alkaline hypochlorite and allowed to develop and grow for two generations on specific RNAi expressing bacterial strains before being used or lifespan analyses
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